Sample Preparation

GoldiSol Price per Box of 500mL Product Code GTS05
1	2+
£125.00	£115.00

AquiGest Price per Box of 500mL Product Code ATS05
1	2+
£90.00	£80.00

INERT SUPPORTS

Procedure
Step	TLC-scrapings		Polyacrylamide gels	TCA precipitates	Filters
#1	Add water soluble samples on TLC silica to 1.0ml water		Add gel to 0.5ml GoldiSol	Add 100mg of dried TCA to 0.2ml water	Stand filter in mini-vial
#2	Stand for 3-5hrs		Stand for 3-5hrs at 50⁰C	Rehydrate for 30mins	Rehydrate with 0.2ml water
#3	Add 10ml ProSafe SE		Add 10ml ProSafe TS	Add 1.0ml GoldiSol	Stand for 30mins at ambient
#4				Stand for 30mins at ambient	Add 5ml ProSafe SE
#5				Add 10ml of ProSafe TS
Cap.			1-2mm gel slice	Up to 100mg
³H eff			45-50%	45-50%	45-50%

BIOLOGICAL

TISSUE

Procedure
Step	Homogenate		Coarse-ground tissue	Bacteria and cells	Organs		Faeces
#1	Add 0.2ml of up to 10% tissue homogenate (in either water or 70% ethanol)		Add 150mg to 2.0ml GoldiSol and swirl	Add 1.0ml of 8:2 GoldiSol:water and swirl	Add 1.0ml GoldiSol to:		Add 0.1ml water to 20mg of faeces (dried) rehydrate for 30mins
					Arteries Brain Liver Cartilage Muscle Cornea Nerve Cells Heart Pancreas Intestine Spleen Kidney Stomach	30-100mg 80-130mg 80-100mg 20-55mg 50-100mg 40-160mg 50-100mg 100-120mg 80-100mg 80-100mg 50-140mg 80-100mg 80-100mg
#2	Add 10ml ProSafe SE		Stand for 3-5hrs at 50⁰C	Stand for 2-4hrs at 500C	Stand for 2-4hrs at 50⁰C		Add 1.0ml GoldiSol and swirl
#3	Shake vigorously		Add 10ml ProSafe TS	Add 10ml ProSafe TS	Add 0.2ml of 30% H₂O₂ and swirl		Stand for 1-2hrs at 50⁰C; Add 0.5ml of IPA, mix; Stand for 2hrs at 50⁰C
#4					Add 10ml ProSafe TS		Add 0.2ml of 30% H₂O₂ and swirl
#5							Stand for 10mins at ambient; Add 10ml ProSafe TS
Cap.	Up to 0.2ml of 10% homogenate		Up to 150mg	Up to 1.0ml
³H eff	35-45%		35-45%	35-40%

BIOLOGICAL FLUIDS AND BUFFERS

Procedure
Step	Blood		Plasm or Serum	Red Blood Cells	Urine	PBS	Aqueous Protein Solution	Sucrose Solution	Inulin fluids	TCA supernatant
#1	Add to 1.0ml GoldiSol		Add 0.5-1.0ml to ProSafe TS	Add 0.2ml 50% RBC suspension + 1.0ml GoldiSol	Add 1.0ml to 10ml ProSafe TS, shake well	Add up to 5.0ml of 10mM PBS to 10ml ProSafe HC	Add 0.1-0.2ml to 1.0ml GoldiSol	Add 3-7ml of 20-40% (w/v) sucrose to 10ml ProSafe HC	Add 50μl to 0.5ml of GoldiSol and shake	Add up to 2.5ml TCA supernatant to 10ml ProSafe HC
#2	Stand for 15-30mins at 50⁰C		Shake until Clear	Stand for 15-30mins at 50⁰C			Stir until dissolved		Add 10ml ProSafe TS
#3	Add 0.3-0.5ml of 30% H₂O₂			Add 0.5ml of 30% H₂O₂			Add 10ml ProSafe TS
#4	Stand for 15-30mins at ambient or 15mins at 50⁰C			Stand for 15-30mins at ambient or 15mins at 50⁰C
#5	Add 10ml of ProSafe TS			Add 10ml of ProSafe TS
Cap.	Up to 0.4ml		Up to 1ml	Up to 0.2ml	Up to 1.0ml	Up to 5.0ml	Up to 0.2ml	Up to 7ml	Up to 50μl	Up to 2.5ml
³H eff	30-35%		30-40%	30-35%	30-40%	30-40%	35-50%	30-40%	50-60%	25-40%